Complex Carbohydrates Part E by Victor Ginsburg (Eds.) PDF

By Victor Ginsburg (Eds.)

ISBN-10: 0121820386

ISBN-13: 9780121820381

The severely acclaimed laboratory ordinary, Methods in Enzymology, is among the so much hugely revered courses within the box of biochemistry. on the grounds that 1955, every one quantity has been eagerly awaited, often consulted, and praised by means of researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate at the present time - really a vital ebook for researchers in all fields of lifestyles sciences

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Additional resources for Complex Carbohydrates Part E

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The neuraminic acid derivatives give abundant MH +, (MH - 32) +, and ~MH - 64) + fragments from loss of one and two molecules of MeOH from the pseudomolecular ion. These are listed, along with retention data on DB-5, in Table 11. Spectra for the terminal and 8-O-substituted NeuNAc derivatives in methane C1 have been published. 3~ It is outside the scope of this article to give extensive listings of retention data for PMAAs. Given the variation in columns, conditions, and temperature programs used by different laboratories, there is no pressing need to publish here yet another tabulation of retention indices.

2] MICROSCALE METHYLATION ANALYSIS OF GLYCOLIPIDS 13 [2] Microscale M e t h y l a t i o n Analysis of Glycolipids Using Capillary Gas C h r o m a t o g r a p h y - C h e m i c a l Ionization Mass F r a g m e n t o g r a p h y with Selected Ion Monitoring By STEVEN B. LEVERY and SEN-ITIROH HAKOMORI Unambiguous information regarding the position of glycosidic linkages and the position of branching in complex carbohydrates and polysaccharides can only be obtained by methylation analysis. The current methodology is based on (1) an effective methylation method using the Corey-Chaykovsky base, L2 and (2) gas chromatography-mass spectrometric (GC-MS) identification of partially O-methylated hexitols or hexosaminitols.

Hakomori, E. Nudelman, S. B. Levery, and R. Kannagi, J. Biol. Chem. 259, 4672 (1984). METHODS IN ENZYMOLOGY, VOL. 138 Copyright © 1987 by Academic Press, Inc. All rights of reproduction in any form reserved. 14 ANALYTICAL METHODS [2] tailed here is the practical procedure that has been used in these studies, which currently allows us to determine routinely all intersugar linkages of a 10-20 nmol sample of glycolipid (allowing for multiple sample injection and reduced yield of polysubstituted N-acetylhexosamines) using bonded-phase fused silica capillary columns and methane chemical ionization mass spectrometry (CI-MS) with selected ion monitoring (SIM).

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Complex Carbohydrates Part E by Victor Ginsburg (Eds.)


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