By Sidney Fleischer, Becca Fleischer (Eds.)
The seriously acclaimed laboratory typical, Methods in Enzymology, is among the so much hugely revered guides within the box of biochemistry. due to the fact that 1955, each one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate this present day - really an important book for researchers in all fields of existence sciences
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Additional info for Biomembranes Part T
This large image ensures that there will be good video resolution since several video scan lines will lie within the diffraction border surrounding the cell. s can be used to provide additional edge detection capability or image expression of structures inside the cell). As mentioned above, it is important to optimize the optical video image; but even with optimization, diffraction at edges of the cell limits the accuracy of measurement of the whole cell geometry. Such limitations can lead to measurement errors of 10-20% for parameters such as surface area and volume of cells.
Z~ R. Waugh and E. Evans, Biophys. J. 26, 115 (1979). G. B. Nash and H. J. Meiselman, Ann. Y. Acad. Sci. 416, 255 (1983). 0 Ap. Rp/2y. FIG. 9. The slope of the pipet suction pressure versus projection length of the cell shown in Fig. 8 is used to derive the membrane extensional rigidity. The pressure "loading" and "unloading" phases are reversible, which demonstrates the elastic memory of the cell membrane. This experiment is only valid over the range of aspiration lengths where the membrane surface remains smooth and does not fold or buckle.
The critical pressure at which the cell buckles or folds is a direct m e a s u r e of the m e m b r a n e bending stiffness or curvature elastic modulus. 0 Pipet Radius (xl0~cm) FIG. 13. The pressures necessary to initiate buckling and subsequent folding in pipet aspiration of flaccid red cells are shown as functions of pipet radius. Correlation of the theoretical prediction of buckling instability with experiment yields the upper bound on the bending stiffness or curvature elastic modulus (B) of the red cell membrane.
Biomembranes Part T by Sidney Fleischer, Becca Fleischer (Eds.)