Download PDF by Sidney Fleischer, Lester Packer: Biomembranes - Part D: Biological Oxidations

By Sidney Fleischer, Lester Packer

ISBN-10: 0076687953

ISBN-13: 9780076687954

ISBN-10: 0121819531

ISBN-13: 9780121819538

The severely acclaimed laboratory normal, Methods in Enzymology, is among the such a lot hugely revered courses within the box of biochemistry. considering that 1955, every one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable this present day - actually a vital booklet for researchers in all fields of lifestyles sciences

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Sidney Fleischer, Lester Packer's Biomembranes - Part D: Biological Oxidations PDF

The severely acclaimed laboratory usual, equipment in Enzymology, is among the such a lot hugely revered courses within the box of biochemistry. considering that 1955, every one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless proper at the present time - actually a necessary ebook for researchers in all fields of existence sciences

Extra info for Biomembranes - Part D: Biological Oxidations

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63, 403 (1956). " K. Minnaert, Biochim. Biophys. Acta 50, 23 (1961). ,2 E. T. McGuinness and W. W. Wainio, J. Biol. Chem. 237, 3273 (1962). ':~T. Yonetani and G. S. Ray, J. Biol. Chem. 240, 3392 (1965). ,4 B. Errede, G. P. Haight, and M. D. Kamen, Proc. Natl. Acad. Sci. A. 73, 113 (1976). "~S. Ferguson-Miller, D. L. Brautigan, a~nd E. Margoliash, J. Biol. Chem. 251, 1104 (1976). '~ H. Lineweaver and D. Burk, J. A m . Chem. Soc. 56, 658 (1934). ,7 p. Nicholls, Biochim. Biophys. Acta 346, 261 (1974).

Chem. 237, 1681 (1962). 2 j. S. Rieske, this series, Vol. 10 [44]. '~ J. S. Rieske, Biochim. Biophys. Acta 456, 195 (1976). 36 ELECTRON TRANSFER COMPLEXES [7] tion, the olive-green precipitate contains complex I, and the red supernatant contains complex III. This supernatant is collected and fractionated as follows. It is important that the supernatant be kept at ice-bath temperature and fractionated without delay and that the centrifuge rotors be precooled to near 0 °. 065 volume) of neutralized saturated ammonium sulfate (saturated at 25°).

C. Salerno, D. B. Winter, J. Lim, C. A. Yu, L. Yu, and T. E. King, J. Biol. Chem. 2S1, 2094 (1976). T. Ohnishi, J. Lim, D. B. Winter, and T. E. King, J. Biol. Chem. 2ill, 2105 (1976). 3 b " From W. G. Hanstein, K. A. Davis, M. A. G h a l a m b o r , and Y. Hatefi, 10, 2517 (1971). 65 mM PMS. (mg protein) -1 at 38 °. a At 3 mM ferricyanide. e Moles of s u c c i n a t e oxidized min -1 (mole e n z y m e ) -1 at 38 °. Biochemistry (PMS) and 2,6-dichloroindophenol (DCIP) is 10-11 × 103. The same value is obtained for the turnover number of succinate dehydrogenase in complex II and in submitochondrial particles treated with 2-thenoyltrifluoroacetone (TTFA) to inhibit ubiquinone reduction.

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Biomembranes - Part D: Biological Oxidations by Sidney Fleischer, Lester Packer


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