By Colin R. Howard, Howard
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Extra info for Arenaviruses (Developments in Biochemistry)
3 for up to 20 h. Subsequent freezedrying of the extract produced an antigen source suitable for shipment to other laboratories as a diagnostic reagent. The material remained stable in a freeze-dried state for up to one month a t 4"C, providing the CF antigen was resuspended into buffer containing bovine albumin. Antibodies against Lassa virus may be detected by immunofluorescence in acute infection from 7 days after onset of illness (Wulff and Lange, 1975) and this is currently the method of choice for the detection 33 of specific antibody.
The name refers to the mountain valley where the isolates were made, and this is described by Trapido and Sanmartin as an upland area covered extensively by secondary forest resulting from the production of regenerating small plants. Much of this area is referred to as fog forest and is a major catchment area for the supply of water to the city of Cali and surrounding areas. The authors found that Pichinde virus could be isolated in the 19 laboratory equally effectively in infant mice, infant hamsters and Vero cell cultures, and positive isolations were made from many organs of trapped animals including the brain, liver, spleen and kidney.
1976) using animals trapped in Georgia found that newborn cotton rats never excreted virus in the urine although infectious virus was present in many tissues. Adult animals had little or no virus present either in blood or in organs. These discrepancies may in part be due to host factor variation within the same species of rodent. For example, Webb et al. (1 973) found that C. laucha caught in Venezuela did not support either acute or chronic Junin infection, despite the fact that this same rodent species represents a major natural reservoir of Junin virus in Argentina.
Arenaviruses (Developments in Biochemistry) by Colin R. Howard, Howard