By Michael Butler
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Additional info for Animal Cell Culture and Technology (THE BASICS (Garland Science))
Spinner bottles can be designed up to a capacity of 5–10 liters. Cultures above this volume require a top-driven motor for stirring. The spinner bottles are suited for growing suspension cells although they can be adapted for anchorage-dependent cells by the use of microcarriers. The spinner flasks are usually siliconized to prevent undue attachment of cells to the inner glass surface. This may be performed by application of dimethyldichlorosilane (Repelcote from Sigma). An appropriately designed stirring base is required for operation of the spinner bottles.
G. traces of heavy metal) into the cultures and would likely result in a cytotoxic effect causing lowered cell growth or an altered metabolism. It is necessary to soak all re-usable glassware in a hypochlorite solution (Chloros) as soon as possible after use. This will prevent the possibility of a build-up of dried protein residues that are difficult to remove. It is desirable to have access to a sink deep enough to accommodate all the dirty glassware at one time and to allow the complete immersion of the largest glass items.
This is useful for the maintenance of a master stock of important cells; ■ tests for contamination in cell lines; ■ characterization of cell lines. This includes isoenzyme analysis, karyotyping and DNA fingerprinting (see Chapter 5). , Agostini, E. and Alema, S. (1987) The v-myc oncogene is sufficient to induce growth transformation of chick neuroretina cells. Nature 326:188–190. D. T. (1952) Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res.
Animal Cell Culture and Technology (THE BASICS (Garland Science)) by Michael Butler